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Ex vivo expansion of hematopoietic stem cells derived from umbilical cord blood in rotating wall vessel.
- Abstract:
Expansion of umbilical cord blood mononuclear cells (UCB MNCs) was carried out in a rotating wall vessel (RWV) bioreactor and tissue culture flasks (T-flasks) in serum-containing medium supplemented with relatively low doses of purified recombinant human cytokines (5.33 ng/ml IL-3, 16 ng/ml SCF, 3.33 ng/ml G-CSF, 2.13 ng/ml GM-CSF, 7.47 ng/ml FL and 7.47 ng/ml TPO) for 8 days. The cell density, pH and osmolality of the culture medium in the two culture systems were measured every 24h. Flow cy...
- Publication status:
- Published
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APA Style
Liu, Y., Liu, T., Fan, X., Ma, X., & Cui, Z. (2006). Ex vivo expansion of hematopoietic stem cells derived from umbilical cord blood in rotating wall vessel. Journal of Biotechnology, 124(3), 592–601.
MLA Style
Liu, Y., et al. “Ex Vivo Expansion of Hematopoietic Stem Cells Derived from Umbilical Cord Blood in Rotating Wall Vessel.” Journal of Biotechnology, vol. 124, no. 3, 2006, pp. 592–601.
Chicago Style
Liu, Y, T Liu, X Fan, X Ma, and Z Cui. 2006. “Ex Vivo Expansion of Hematopoietic Stem Cells Derived from Umbilical Cord Blood in Rotating Wall Vessel.” Journal of Biotechnology 124 (3): 592–601.
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- Publisher copy:
- 10.1016/j.jbiotec.2006.01.020
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Authors
+ Liu, Y More by this author + Liu, T More by this author + Fan, X More by this author + Ma, X More by this author + Cui, Z More by this author
Bibliographic Details
- Journal:
- Journal of biotechnology More from this journal
- Volume:
- 124
- Issue:
- 3
- Pages:
- 592-601
- Publication date:
- 2006-07-01
- DOI:
- EISSN:
-
1873-4863
- ISSN:
-
0168-1656
Item Description
- Language:
- English
- Keywords:
-
Cell Culture Techniques
Equipment Failure Analysis
Cell Count
Tissue Engineering
Rotation
Fetal Blood
Feasibility Studies
Equipment Design
Cell Proliferation
Humans
Cells, Cultured
Mechanotransduction, Cellular
Bioreactors
Hematopoietic Stem Cells
- Pubs id:
-
pubs:62031
- UUID:
-
uuid:f84c816b-0c67-4c4f-8857-b912b4a2449d
- Local pid:
- pubs:62031
- Source identifiers:
-
62031
- Deposit date:
- 2013-11-17
Terms of use
- Copyright date:
- 2006
- Licence:
- Terms and Conditions of Use for Oxford University Research Archive
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